Application of Fish Collagen-Nanochitosan-Henna Extract Composites for the Control of Skin Pathogens and Accelerating Wound Healing

Skin is the largest protective organ that could be recurrently wounded and attacked by microorganisms. The skin safeguarding supporting were intended through natural derivatives. Fish collagen (Cg) type I, extracted from sea bream (Spondyliosoma cantharus), chitosan nanoparticles (NCht) shrimp shells, henna (Lawsonia inermis L.) leaves extract (He) produced physiochemically characterized. antimicrobial potentialities of these compounds their composites assessed toward pathogens (Candida albicans Staphylococcus aureus) using various assaying methods microimaging techniques. infrared electrophoretic analysis Cg validated its characteristics, IR-spectroscopic compounds/composites indicated physiochemical attributes interrelations. NCht particles had a diameter range 64.6-308.8 nm, 104 nm mean diameter, +31.3 mV zeta potentiality. Both NCht, He, NCht/He composite exhibited significant potentiality pathogens; was strongest with inhibitory concentrations 20.0 22.5 μg/mL inhibition zones 25.7 26.8 mm against S. aureus C. albicans, respectively. electron micrographs verified synergistic microbicidal action NCht/He, as they led to severe microbial lysis deformations. wounds’ treatment NCht/He/Cg promoted fastest complete healing rats’ during 8 days local treatment, absence inflammation infection signs; treated composite, wound area vastly reduced 63.6 mm2 15.9 9.1 after 2 4 days, are recommended topical applications for optimum disinfection regeneration.